Advanced Cell Diagnostics

Provider of the most advanced
RNA in situ hybridization technology
Novel RNA Hybridization Method for the In Situ Detection of ETV1, ETV4, and ETV5 Gene Fusions in Prostate Cancer, Kunju LP, Carskadon S, Siddiqui J, Tomlins SA, Chinnaiyan AM, Palanisamy N., Appl Immunohistochem Mol Morphol., 2014 September 09 --- Hyperplasia and fibrosis in mice with conditional loss of the TSC2 tumour suppressor in Müllerian duct mesenchyme-derived myometria, Kaneko-Tarui T, Commandeur AE, Patterson AL, DeKuiper JL, Petillo D, Styer AK, Teixeira JM., Mol Hum Reprod., 2014 September 04 --- Characterization of Inflammatory (Lymphoepithelioma-like) Hepatocellular Carcinoma: A Study of 8 Cases., Patel KR, Liu TC, Vaccharajani N, Chapman WC, Brunt EM., Arch Pathol Lab Med., 2014 September 02 --- Axonally Synthesized ATF4 Transmits a Neurodegenerative Signal across Brain Regions, Baleriola J, Walker CA, Jean YY, Crary JF, Troy CM, Nagy PL, Hengst U., Cell, 2014 August 28 --- The Raccoon Polyomavirus Genome and Tumor Antigen Transcription are Stable and Abundant in Neuroglial Tumors., Brostoff T, Dela Cruz FN Jr, Church ME, Woolard KD, Pesavento PA., J Virol. , 2014 August 27 --- Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos , Gross-Thebing T, Paksa A, Raz E., BioMed Central, 2014 August 15 --- Simultaneous flow cytometric analysis of IFN-γ and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation., Van Hoof D, Lomas W, Hanley MB, Park E., Cytometry A., 2014 August 13 --- Effect of local application of an antibody against brain-derived neurotrophic factor on neuroma formation after transection of the inferior alveolar nerve in the rat., Valverde Guevara YM, Yoshikawa H, Saito I, Maeda T, Seo K., Neuroreport, 2014 July 22 --- Temporal control over the initiation of cell motility by a regulator of G-protein signaling., Hartwig J, Tarbashevich K, Seggewiß J, Stehling M, Bandemer J, Grimaldi C, Paksa A, Groß-Thebing T, Meyen D, Raz E., Proceedings of the National Academy of Sciences of the United States of America, 2014 July 21 --- A Novel Chromogenic In Situ Hybridization Assay for FGF23 mRNA in Phosphaturic Mesenchymal Tumors., Carter JM, Caron BL, Dogan A, Folpe AL., American Journal of Surgical Pathology, 2014 July 14 ---


Delivers Quantitative Molecular Detection and Morphological Context (MD & MC) in a Single Assay

RNAscope® Technology is a cutting-edge multiplex nucleic acid in situ hybridization technology, based on ACD’s unique, patented probe design strategy which enables simultaneous signal amplification and background noise suppression.  RNAscope Technology represents one of the most significant advances in ISH technology in over 40 years.

What is the value of Quantitative Molecular Detection and Morphological Context (MD & MC) in a single assay? What makes RNAscope unique?

  • Molecular Detection: RNAscope detects single RNA molecules and provides quantitative analysis of gene expression at the single-cell resolution.
  • Morphological Context: RNAscope localizes RNA molecules providing spatial and cell-specific expression while preserving tissue architecture.
  • Quantitative: RNAscope quantifies RNA molecules per cell, giving accurate expression level at the single cell resolution across heterogeneous cell populations. 
  • Universal:  RNAscope's patented probe design can be applied to virtually ANY gene in ANY genome in ANY tissue.
  • Scalable: The assay is robust and easy to implement in basic, translational and clinical research labs. Availability of Manual Assays, Automated Assays and Assay Services provide ultimate flexibility.

RNAscope enables in situ detection of RNA biomarkers—filling a long standing gap in in situ molecular analysis.

RNAscope can complement IHC when antibodies are unavailable or have insufficient sensitivity or uncertain specificity. RNAscope is ideal for detecting RNAs for secreted proteins and non-coding RNA—a limitation of IHC.

Downstream of microarrays and NGS (e.g., RNA-seq), RNAscope is an ideal platform for translating new scientific advances into clinical applications due to the speed of RNAscope assay development and its superior sensitivity and specificity.

Other RNA biomarker analysis methods such as RT-PCR can provide quantitative, sensitive and specific measurements of RNA transcripts. However, the resulting quantitative gene expression information is without morphological and cellular context and only represents a “group average” of an admixture of heterogeneous populations of cells. RNAscope’s ability to provide gene expression at the single cell resolution coupled with morphological context is well-suited for RNA analysis in tissue diagnostics applications.